![]()
Brand AVX TPSE226M035R0125 Low impedance tantalum capacitor AVX 22
![]()
Electronic scale crystal oscillator 3.2*2.5mm 3225 16M (16.000MHZ) 12PF 10PPM 20PPM 30PPM
![]()
Huaqiang Square crystal original spot stock
Purpose of usage:
This kit is designed to detect the presence of Salmonella in swine serum, plasma, and other related fluid samples. The method used is a double-antibody sandwich ELISA, which allows for highly specific and sensitive detection of porcine salmonellosis.
Experimental Principle:
The kit utilizes a solid-phase antibody coated on a microplate, which binds to the target antigen present in the sample. After washing away unbound substances, a horseradish peroxidase (HRP)-labeled secondary antibody is added, forming an antibody-antigen-enzyme complex. A colorimetric reaction occurs when TMB substrate is introduced, changing from blue to yellow under the action of an acidic stop solution. The optical density (OD) at 450 nm is measured using a microplate reader, and results are interpreted based on a pre-established cutoff value.
Kit Composition:
- 30x Wash Solution: 20ml × 1 bottle
- Stop Solution: 6ml × 1 bottle
- Enzyme Standard Reagent: 6ml × 1 bottle
- Positive Control: 0.5ml × 1 bottle
- Enzyme-Labeled Coating Plate: 12 wells × 8 strips
- Negative Control: 0.5ml × 1 bottle
- Sample Diluent: 6ml × 1 bottle
- Developer A: 6ml × 1 bottle
- Developer B: 6ml × 1 bottle
- Instructions: 1 set
- Sealing Film: 2 sheets
- Seal Bag: 1 piece
Sample Requirements:
Samples should be processed as soon as possible after collection, following appropriate protocols. If testing is delayed, store samples at -20°C, avoiding repeated freeze-thaw cycles. Note that NaN3-containing samples cannot be tested, as it inhibits HRP activity.
Operation Steps:
1. Label the microwells according to the sample number.
2. Add 50 μL of negative and positive controls to their respective wells. Add 40 μL of diluent and 10 μL of sample to each test well.
3. Cover with a sealing film and incubate at 37°C for 30 minutes.
4. Prepare the 30x wash solution by diluting with distilled water.
5. Wash the plate 5 times with the diluted solution, ensuring thorough removal of unbound material.
6. Add 50 μL of enzyme-labeled reagent to each well except the blank.
7. Incubate again at 37°C for 30 minutes.
8. Repeat the washing steps.
9. Add 50 μL of TMB A and 50 μL of TMB B, mix gently, and incubate at 37°C for 10 minutes in the dark.
10. Add 50 μL of stop solution to each well to terminate the reaction.
11. Measure OD values at 450 nm within 15 minutes of stopping the reaction.
Calculation and Result Interpretation:
- Validity Check: Positive control OD ≥ 1.00; Negative control OD ≤ 0.10
- Cutoff Value = Negative Control Average + 0.15
- Results: OD < Cutoff = Negative; OD ≥ Cutoff = Positive
Notes:
- Follow instructions carefully; do not mix reagents from different batches.
- Allow the kit to reach room temperature before use. Store unused strips in sealed bags.
- The wash solution may crystallize but will not affect results if properly dissolved.
- Use the sealing film only once to prevent contamination.
- Keep substrates away from light.
- Results must be read using a microplate reader. For dual-wavelength detection, use 630 nm as reference.
- Treat all samples, washes, and waste as biohazardous materials. Handle stop solution (2M sulfuric acid) with care.
Storage Conditions:
- Store at 2–8°C.
- Shelf life: 6 months.
Solar Mounting System
Solar Mounting System,Solar Water Panel Stand,solar panel metal ground mount,ground mounted solar
Hebei Jinbiao Construction Materials Tech Corp., Ltd. , https://www.pvcarportsystem.com