Digital Multimeter ,Bench Type Digital Multimeter,Meco Multimeter,Hioki Multimeter YINTE TOOLS (NINGBO) CO., LTD , https://www.yinte-tools.com
Digital Multimeter ,Bench Type Digital Multimeter,Meco Multimeter,Hioki Multimeter YINTE TOOLS (NINGBO) CO., LTD , https://www.yinte-tools.com
Recently, many of our customers have reached out to our sales team with questions about the ELISA kit. To help you better understand how to use it properly, we’ve compiled a detailed guide based on common issues and best practices. We hope this will make your experience smoother and more accurate:
1. Before using the ELISA kit, take it out from the refrigerator and let it reach room temperature for 15–30 minutes. If the enzyme label is opened, store the remaining strips in a sealed bag to maintain their integrity.
2. The concentrated washing solution may crystallize when stored. When diluting, gently heat it in a water bath until fully dissolved. This won’t affect the final test results.
3. Always use a dedicated pipette for each step, and recalibrate regularly to ensure accuracy. Try to complete each step within 5 minutes. For large sample volumes, using a multichannel pipette is highly recommended.
4. It’s essential to create a standard curve every time you perform the test, and ideally, run duplicate wells. If the sample’s OD value exceeds that of the first standard well, dilute the sample before testing. Remember to multiply the final result by the dilution factor (e.g., ×n×5) when calculating.
5. The sealing film is for single-use only to prevent cross-contamination between different samples or wells.
6. Keep the substrate away from direct light to preserve its stability and performance.
7. Always follow the instructions provided in the manual. The final results should be read using a microplate reader, not just visual inspection.
8. Treat all samples, wash solutions, and waste materials as potentially infectious. Proper disposal is crucial for safety.
9. Do not mix components from different batches of the reagent, as this can lead to inconsistent results.
10. In case of discrepancies between the English and other language manuals, the English version takes precedence.
If you have any further questions or need clarification, feel free to contact us. We’re here to help and ensure your ELISA experiments are both accurate and efficient. EL-C1600N100013-B Spot Remarks 2017+ Test - lowercase jpg