ELISA kits are widely available in the market and are highly favored by researchers due to their high reproducibility, strong heterogeneity, and high sensitivity. With so many options on the market, it can be overwhelming for users to choose the right one. Many people suffer from selection syndrome—unsure of which ELISA kit is user-friendly or how to evaluate its quality. As a result, they may end up choosing an unsuitable product. To help you make an informed decision, our company has compiled some common knowledge about ELISA kits. We hope this information will serve as a useful reference when purchasing. Although there are various types of ELISA kits, they all share a common principle: antigen capture. The most typical format involves adsorbing the antigen onto a microplate or capturing it using antibodies on the plate. In-cell ELISA and ELISPOT are two special formats. In-cell ELISA requires cell immobilization and permeabilization within microplates, followed by in situ detection with antibodies. ELISPOT is somewhat similar to Western blotting. Cells are first cultured on membrane-coated plates, and then the antigens secreted by the cells are detected on the membrane, forming visible spots. When conducting ELISA experiments, you should choose between 96-well or 384-well plates based on your specific needs. Both monoclonal and polyclonal antibodies can be used in ELISA kits. Sometimes, combining both can yield better results. For example, in the double-antibody sandwich method, using a polyclonal antibody for capture and a monoclonal antibody for detection often provides improved accuracy. Polyclonal antibodies ensure that all target antigens are captured, while monoclonal antibodies specifically detect those with unique epitopes. Since its establishment, Shanghai Jinma has always been committed to providing sincere service to every customer, guided by an honest and professional business philosophy. Our core value is: Integrity is the foundation of trust, and only through honesty can we achieve long-term success. We deeply appreciate your continued support over the years. Through our ongoing efforts, we aim to make greater contributions to the field of scientific research and continue to provide reliable products and services to our valued customers. Distribution Transformer,Electric Oil Type Distribution Transformer,Oil Type Distribution Transformer,Oil-Immersed Special Transformer Shaoxing AnFu Energy Equipment Co.Ltd , https://www.sxanfu.com