Cell NOS determination and some problems in the instructions? -Huaqiang Electronic Network

Test probe P100-B can be customized for other specifications

Kaixin micro test

Test - lowercase jpg

To measure cells

iNOS

Vitality, which formula should I use? Where can I find the nanomolar extinction coefficient? Operation table

a*

How many,

a*

Does it represent the sample size, how do I calculate the sample size of my cell supernatant?

Reply:

In the cell

i-NOS

It can be calculated according to the processing method of the tissue sample, and it is necessary to introduce the protein concentration in the cells.

Color nanomolar extinction coefficient

It is a fixed value and is the value provided in the manual.

a*

Representing the sample size, generally

30ul

-

100ul

. If the sample to be measured is the supernatant, it needs to be calculated and processed according to the serum and plasma samples.

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