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CCK-8 detection kit - cell activity detection method - Database & Sql Blog Articles
The CCK-8 Test Kit is a widely used method for evaluating cell viability, proliferation, and cytotoxicity. One of its main advantages is that it does not require DMSO to dissolve the reagent, making it non-toxic and safe for use. It is also convenient and efficient, allowing researchers to quickly and accurately measure cell activity without complicated procedures.
This kit is particularly useful in various biological studies, including drug screening, toxicity testing, and cell growth analysis. The operation process is straightforward, ensuring consistent and reliable results. But how exactly does it work, and how do you choose the right CCK-8 test kit for your research?
**CCK-8 Cell Activity Assay**
1. Seed a cell suspension (100 μl/well) into a 96-well plate.
2. Incubate the plate at 37°C with 5% CO₂ for a specific time period.
3. Add 10 μl of CCK-8 solution to each well, being careful not to create bubbles.
4. Incubate for 1–4 hours.
5. Measure the absorbance at 450 nm using a microplate reader.
If the OD value cannot be measured immediately, you can add 10 μl of 0.1 M HCl or 1% SDS to each well and store the plate in the dark at room temperature. The absorbance remains stable for up to 24 hours.
**CCK-8 Cell Proliferation and Toxicity Assay**
1. Seed 100 μl of cell suspension (2000–5000 cells) into a 96-well plate.
2. Incubate for 24 hours at 37°C with 5% CO₂.
3. Add 10 μl of different concentrations of the test substance.
4. Incubate for an appropriate time (e.g., 6, 12, 24, or 48 hours).
5. Add 10 μl of CCK-8 solution to each well.
6. Incubate for another 1–4 hours.
7. Measure the absorbance at 450 nm.
If the test substance is oxidizing or reducing, it's recommended to replace the medium with fresh medium after removing the original one and washing the cells twice. This helps eliminate any interference from the substance. If the effect is minimal, you can subtract the blank absorbance directly after adding the test compound.
**How to Choose the Right CCK-8 Test Kit?**
Currently, there are both domestic and imported CCK-8 kits available on the market. However, domestic products often suffer from poor quality stability, while imported brands may be more expensive. For example, major international brands like Abcam, Thermo, and Sigma do not always offer CCK-8 kits, whereas Abbkine provides high-quality, cost-effective alternatives.
As the flagship product of the Abbkine brand, the **Cell Counting Kit (CCK-8)** has been developed by experienced R&D engineers who have continuously optimized the formula. Their goal is to make scientific research easier, faster, and more reliable for researchers around the world.
Each kit comes with a single tube of CCK-8 solution containing WST-8, eliminating the need for additional preparation. No isotopes are required, and all steps are performed in the same 96-well plate. There’s no need to wash or collect cells, and no extra steps are needed to dissolve formazan. This makes it ideal for high-throughput screening and large-scale sample analysis.
Here’s a quick comparison of some popular CCK-8 kits:
| Brand (Specification) | Abbkine | Sigma | Dojindo |
|-----------------------|---------|-------|---------|
| 100 times | ¥200 | ¥703.17 | ¥350 |
| 500 times | ¥640 | ¥2779.92 | ¥890 |
| 2000 times | ¥2050 | ¥13899.6 (3000 times) | —— |
| 10,000 times | ¥7180 | —— | ¥8380 |
As the official agent of Abbkine in China, Amy Jiejie offers cost-effective immunological reagents, including various tagged antibodies, internal reference antibodies, primary and secondary antibodies, detection substrates, and related kits. Additionally, as an agent for several international brands, Aimei provides comprehensive lab Solutions tailored to your needs.
For more information or assistance with your experiments, feel free to contact us at **400-6800-868** or reach out to **Amytech Co., Ltd.** for detailed product information and full experimental support.