Data Centre,Fiber Optic Patch Cord Assembly,Multimode Fiber Optic Patch Cord,Outdoor Fiber Optic Patch Cord Huizhou Fibercan Industrial Co.Ltd , https://www.fibercan-network.com
CCK-8 detection kit - cell activity detection method - Database & Sql Blog Articles
The CCK-8 test kit is a widely used method for assessing cell viability, proliferation, and cytotoxicity. Unlike traditional methods that require the use of DMSO to dissolve reagents, the CCK-8 kit is non-toxic and easy to handle, making it a safer and more convenient option for researchers. Its simplicity and efficiency make it ideal for high-throughput screening and routine laboratory testing.
One of the key advantages of the CCK-8 assay is its ability to provide accurate and reproducible results without the need for cell lysis or extensive washing steps. The procedure involves adding a small volume of the CCK-8 solution directly to the cell culture, allowing the reaction to proceed in the same 96-well plate. This not only saves time but also reduces the risk of contamination or error during the process.
Here is a step-by-step guide for using the CCK-8 test kit:
**Cell Activity Assay:**
1. Seed a cell suspension (100 μl/well) into a 96-well plate.
2. Incubate the plate at 37°C with 5% CO₂ for an appropriate period.
3. Add 10 μl of CCK-8 solution to each well, being careful to avoid bubble formation.
4. Incubate for 1–4 hours.
5. Measure the absorbance at 450 nm using a microplate reader.
**Cell Proliferation and Toxicity Assay:**
1. Seed 100 μl of cell suspension (2000–5000 cells) into a 96-well plate.
2. Incubate for 24 hours under standard conditions.
3. Add 10 μl of the test substance to each well.
4. Incubate for a suitable time (e.g., 6, 12, 24, or 48 hours).
5. Add 10 μl of CCK-8 solution to each well.
6. Incubate for 1–4 hours.
7. Measure the absorbance at 450 nm.
If the measurement cannot be performed immediately, you can add 10 μl of 0.1 M HCl or 1% SDS to each well, cover the plate, and store it at room temperature in the dark. The absorbance will remain stable for up to 24 hours.
In cases where the test substance is oxidizing or reducing, it is recommended to replace the medium with fresh medium after removing the original one and washing the cells twice. This helps eliminate any interference from the test compound. If the effect is minimal, you can subtract the blank absorption after adding the drug to the medium.
When choosing the right CCK-8 test kit, it's important to consider factors such as quality, stability, and cost-effectiveness. While there are both domestic and imported options available, many users prefer imported brands for their reliability and consistency. However, some international suppliers do not offer CCK-8 kits, making it difficult for researchers to find the best fit for their needs.
Abbkine’s Cell Counting Kit (CCK-8) is a leading product in this field, developed through extensive research and optimization by Abbkine’s R&D team. Each batch is carefully formulated to ensure accuracy, stability, and ease of use. The kit comes with a single tube of CCK-8 solution containing WST-8, eliminating the need for additional preparation. It is compatible with various cell types and can be used for large-scale sample analysis.
For more information on the CCK-8 test kit and other related products, please contact Amytech Co., Ltd. at 400-6800-868. They offer a wide range of immunological reagents, including antibodies, detection substrates, and experimental Solutions, tailored to meet the needs of researchers across different fields.
| Brand (Specification) | Abbkine | Sigma | Dojindo |
|-----------------------|---------|-------|---------|
| 100 times | ¥200 | ¥703.17 | ¥350 |
| 500 times | ¥640 | ¥2779.92 | ¥890 |
| 2000 times | ¥2050 | ¥13899.6 (3000 times) | —— |
| 10,000 times | ¥7180 | —— | ¥8380 |
Whether you're conducting basic research or developing new therapies, the CCK-8 test kit offers a reliable and efficient way to assess cell behavior. With its user-friendly design and proven performance, it has become an essential tool in modern cell biology laboratories.